Adult heart muscle cells: Isolation, properties and applications

Isolated adult heart cells. The development of a new experimental model.- Ca-tolerant guinea-pig ventricular myocytes as isolated by pronase in the presence of 250 ?M free calcium.- Guinea-pig atrial cardioballs.- Determination of isolated myocyte viability: Staining methods and functional criteria.- Sarcolemma-bound calcium. Its importance for cell viability.- Anoxic injury of adult cardiac myocytes.- Non-invasive measurements of cell surface receptors.- Surface antigens of adult heart cells and their use in diagnosis.- Substrate utilization of adult cardiac myocytes.- Characteristics of lactate transfer in isolated cardiac myocytes.- The insulin receptor of adult heart muscle cells.- Kinetics of cellular uptake of tracers used in myocardial scintigraphy.- Adhesion of cardiac myocytes to extracellular matrix components.- Development of new intercellular contacts between adult cardiac myocytes in culture.- Long-term primary cultures of adult human and rat cardiomyocytes.- Morphological dedifferentiation of adult cardiac myocytes in coculture with hepatocytes.- Electrophysiological properties of isolated ventricular myocytes.- The effect of cyanide on the K-current in guinea-pig ventricular myocytes.- Measurements of single-channel currents in the membrane of isolated cells: ATP-dependence of K+-channels.- Evidence for Ca-mediated inactivation of ICa in dialysed guinea-pig atrial cardioballs.- Cardiac membrane currents and energetic state.- Simultaneous measurements of action potentials and contractions in single cultured adult and embryonic heart muscle cells.- The two components in the shortening of unloaded ventricular myocytes: Their voltage dependence.- Temperature dependence of verapamil action.- Microtubules and desmin filaments during the onset of heart growthin the rat.- The correlation between catecholamine and lipid peroxidation induced damage in heart cells.- Anoxia in neonatal rat heart cell cultures.- Enzyme release and glycolytic energy production.- Anoxia influences the lateral diffusion of a lipid probe in the plasma membrane of isolated cardiac myocytes.- Summaries of the discussions of the symposium.- Abstracts of the poster-session on the adult myocyte model.- Author Index.