This book presents a summary of work done by the AGRA task group beginning in 2004. The first phase was identification of the agricultural biotechnology research needs by the relevant regulatory agencies, followed by a portfolio analysis covering research supported by all of the participating Federal agencies. This report presents a "snapshot" of the directed biotechnology risk analysis research supported by Federal funding for this specific time period. In the time since the portfolio analysis was completed, the Federal agencies participating in AGRA have used the outcomes to address risk analysis issues.