Betel leaf is tested to have antioxidant, antimicrobial and bactericidal properties. The antimutagenic and antimicrobial activity of the ethanolic extract of Piper betel leaves was determined based on its intrinsic antioxidant potential. This research deals with antimutagenic and bactericidal effect of betel vine extract. many reports are available on antimicrobial effect of this plants, but very few works are available which showed its antimutagenic proterty and DNA protection/repair ability. The methodology described in this work were very in simplified form and contained details information, which will help the readers to follow this work on other important plants.