Basic Science Methods for Clinical Researchers
Editat de Morteza Jalali, Francesca Yvonne Louise Saldanha, Mehdi Jalalien Limba Engleză Paperback – 3 apr 2017
This book will be a vital companion for clinicians undertaking laboratory-based science. It will support clinicians in the pursuit of their academic interests and in making an original contribution to their chosen field. In doing so, it will facilitate the development of tomorrow’s clinician scientists and future leaders in discovery science.
- Serves as a helpful guide for clinical researchers who lack a conventional science background
- Organized around research themes pertaining to key biological molecules, from genes, to proteins, cells, and model organisms
- Features protocols, techniques for troubleshooting common problems, and an explanation of the advantages and limitations of a technique in generating conclusive data
- Appendices provide resources for practical research methodology, including legal frameworks for using stem cells and animals in the laboratory, ethical considerations, and good laboratory practice (GLP)
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Specificații
ISBN-13: 9780128030776
ISBN-10: 0128030771
Pagini: 382
Dimensiuni: 152 x 229 x 23 mm
Greutate: 0.61 kg
Editura: ELSEVIER SCIENCE
ISBN-10: 0128030771
Pagini: 382
Dimensiuni: 152 x 229 x 23 mm
Greutate: 0.61 kg
Editura: ELSEVIER SCIENCE
Cuprins
1. The Polymerase Chain Reaction: PCR, qPCR, and RT-PCR 2. Methods of Cloning 3. Whole-Mount In Situ Hybridization and a Genotyping Method on Single Xenopus Embryos 4. Microarrays: An Introduction and Guide to Their Use 5. Analysis of Human Genetic Variations Using DNA Sequencing 6. Western Blot 7. The Enzyme-Linked Immunosorbent Assay: The Application of ELISA in Clinical Research 8. Immunofluorescence 9. Cell Culture: Growing Cells as Model Systems In Vitro 10. Flow Cytometry 11. Transfection 12. In Vivo Animal Modeling: Drosophila 13. Zebrafish as a Research Organism: Danio rerio in Biomedical Research 14. Xenopus as a Model Organism for Biomedical Research 15. Basic Mouse Methods for Clinician Researchers: Harnessing the Mouse for Biomedical Research
Recenzii
"The information provided would be an excellent tool for a clinician in training…the content is best suited for those clinicians who lack basic science training or a research background."--Annals of Allergy, Asthma & Immunology