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Biochemistry Laboratory: Modern Theory and Techniques

Autor Rodney F. Boyer
en Limba Engleză Paperback – 31 dec 2010
The biochemistry laboratory course is an essential component in training students for careers in biochemistry, molecular biology, chemistry, and related molecular life sciences such as cell biology, neurosciences, and genetics. Increasingly, many biochemistry lab instructors opt to either design their own experiments or select them from major educational journals. Biochemistry Laboratory: Modern Theory and Techniques addresses this issue by providing a flexible alternative without experimental protocols. Instead of requiring instructors to use specific experiments, the book focuses on detailed descriptions of modern techniques in experimental biochemistry and discusses the theory behind such techniques in detail. An extensive range of techniques discussed includes Internet databases, chromatography, spectroscopy, and recombinant DNA techniques such as molecular cloning and PCR. The Second Edition introduces cutting-edge topics such as membrane-based chromatography, adds new exercises and problems throughout, and offers a completely updated Companion Website.
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Specificații

ISBN-13: 9780136043027
ISBN-10: 013604302X
Pagini: 362
Dimensiuni: 185 x 229 x 18 mm
Greutate: 0.52 kg
Ediția:2
Editura: Prentice Hall

Notă biografică

Rod Boyer served on the faculty at Hope College, Holland, MI, where he taught, researched, and wrote biochemistry for 26 years. He earned his B.A. in chemistry and mathematics at Westmar College (Iowa) and his Ph.D. in physical organic chemistry at Colorado State University. After three years as an NIH Postdoctoral Fellow with M. J. Coon (cytochromes P-450) in the Department of Biological Chemistry at the University of Michigan Medical School, he joined the chemistry faculty at Hope. There he directed the work of over 75 undergraduate students in research supported by the NIH, NSF, Dreyfus Foundation, Howard Hughes Medical Institute, and the Petroleum Research Fund (ACS). With his students, he published numerous journal articles in the areas of ferritin iron storage and biochemical education. He spent a sabbatical year as an American Cancer Society Scholar in the lab of Nobel laureate, Tom Cech at the University of Colorado, Boulder. Rod is also the author of Modern Experimental Biochemistry (Third Edition, 2000, Benjamin-Cummings) and Concepts in Biochemistry (Third Edition, 2006, John Wiley & Sons) and serves as an Associate Editor for the journal Biochemistry and Molecular Biology Education (BAMBED). He is a member of the American Society for Biochemistry and Molecular Biology (ASBMB) and a former member of its Education and Professional Development Committee that recently designed the undergraduate biochemistry degree recommended by the ASBMB. Rod now resides in Bozeman, Montana, where he continues to write and consult in biochemical education.

Cuprins

Chapter 1: Introduction to the Biochemistry/Molecular Biology Laboratory Chapter 2: Using the Computer and Internet for Research in Biochemistry Chapter 3: General Laboratory Procedures Chapter 4: Centrifugation Techniques in Biochemistry/Molecular Biology Chapter 5: Purification and Analysis of Biomolecules by Chromatography Chapter 6: Characterization of Proteins and Nucleic Acids by Electrophoresis Chapter 7: Spectroscopic Analysis of Biomolecules Chapter 8: Biomolecular Interactions: Ligand Binding and Enzyme Reacations Chapter 9: Molecular Biology I: Structures and Analysis of Nucleic Acids Chapter 10: Molecular Biology II: Recombinant DNA, Molecular Cloning and Enzymology Chapter 11: Protein Production, Purification, and Characterization Appendices Index