Biophysics is an interdisciplinary science which focuses on studying the phenomena related to various biological systems using the principles and methods of physics. It covers the entire spectrum of biological organizations ranging from biomolecules to entire ecosystems. Molecular biophysics seeks to address the questions related to molecular biology and biochemistry. It makes use of several imaging techniques such as crystallography, spectroscopy, electron microscopy and small angle scattering for visualizing biological structures. Molecular biophysics draws on the principles of thermodynamics, chemical kinetics and statistical mechanics to study complex biological events. Some major areas of research under this field are neuroscience, bioengineering, DNA structure and analysis, computer modeling and medical physics. This book is compiled in such a manner, that it will provide in-depth knowledge about the theory and practice of biological physics. Most of the topics introduced herein cover new techniques and the applications of this discipline. Through this book, we attempt to further enlighten the readers about the new concepts in this field.