This report, "Bioremediation in the Highway Environment: Three Case Studies", was prepared to provide an update and assist the Federal Highway Administration's Priority Technology Program (PTP). It is intended to identify barriers that hinder the widespread use of bioremediation and methods to overcome these barriers and to act as a resource for Departments of Transportation (DOT) in all 50 states. The activities reported here were conducted based on design, construction, performance, and quality assurance information outlined in the HITEC and EvTEC Protocols.