Chemical pesticides and bioinsecticide alone or there mixture, are immunotoxic to earthworm, fish, and mice. The study consisted of three parts: A- the development of a new ultrasound protocol for extrusion of coelomocytes from the earthworm, B- comparative cellular responses of earthworm coelomocytes, of different ages and from different worm species,exposed in vitro to the tested pesticides; C- evaluation of the immunotoxicity of the mixture of the tested pesticides on three animal models (earthworm, Eisenia fetida; trout fish, and inbred female C57BI/6 mice , From an immunological point of view, the chemical pesticides mixture, at the tested levels may not pose a grater risk to the immune system in fish and mic