Biotechnology Proteins to PCR: A Course in Strategies and Lab Techniques

1. Introduction to the Biotechnology Laboratory.- 1.1Overview.- 1.2 Background.- 1.3 Experimental Design and Procedures.- Study Questions.- Further Readings.- 2. Introduction to Proteins.- 2.1 Overview.- 2.2 Background.- 2.3 Experimental Design and Procedures.- Biuret Protein Assay.- Lowry Protein Assay.- Study Questions.- Further Readings.- 3. Protein Isolation and Preparation of Crude Extract.- 3.1 Overview.- 3.2 Background.- 3.3 Experimental Design and Procedures.- Study Questions.- Further Readings.- 4. Batch Purification of Proteins.- 4.1 Overview.- 4.2 Background.- 4.3 Experimental Design and Procedures.- Study Questions.- Further Readings.- 5. Protein Purification by Column Chromatography.- 5.1 Overview.- 5.2 Background.- 5.3 Experimental Design and Procedures.- Study Questions.- Further Readings.- 6. Protein Analysis and Verification.- 6.1 Overview.- 6.2 Background.- 6.3 Experimental Design and Procedures.- Study Questions.- Further Readings.- 7. Designing a Cloning Scheme.- 7.1 Overview.- 7.2 Background.- 73 Experimental Design and Procedures.- 8. Isolation and Preparation of Nucleic Acids.- 8.1 Overview.- 8.2 Background.- 8.3 Expermental Design and Procedures.- Study Questions.- Further Readings.- 9. Constructing a Gene Bank.- 9.1 Overview.- 9.2 Background.- 9.3 Experimental Design and Procedures.- Study Questions.- Further Readings.- 10. Introducing Recombinant Molecules into Escherichia coli.- 10.1 Overview.- 10.2 Background.- 10.3 Experimental Design and Procedures.- Study Questions.- Further Readings.- 11. Screening for Clones.- 11.1 Overview.- 11.2 Background.- 11.3 Experimental Design and Procedures.- Study Questions.- Further Readings.- 12. Characterizing and Verifying Cloned DNA.- 12.1 Overview.- 12.2 Background.- 12.3 Experimental Design and Procedures.- Study Questions.- Further Readings.- 13. Application of DNA Sequence and Clone Data.- 13.1 Overview.- 13.2 Background.- 13.3 Experimental Design and Procedures.- Study Questions.- Further Readings.- 14. The Value of Information.