Cell–Penetrating Peptides – Design, Development and Applications

Prof. Makoto Oba received his Ph.D. degree in pharmaceutical sciences from Kyushu University in 2004. He joined the faculty of the University of Tokyo (2004-2010) and Nagasaki University (2010-2020). He moved to Kyoto Prefectural University of Medicine on 2020 as full Professor at Graduate School of Medicine. He has authored over 100 scientific publications and has received several awards, including the Pharmaceutical Society of Japan Award for Young Scientists (2013), Nagasaki Prefectural Science and Technology Award for Young Scientists (2016), and the Incentive Award in Synthetic Organic Chemistry (2019). Dr. Yosuke Demizu received his Ph.D. in pharmaceutical sciences from Kyushu University in 2006. He joined the faculty of Tokushima Bunri University (2004-2005) and then at Nagasaki University (2006-2008). He moved to the National Institute of Health Sciences (NIHS) as a researcher, and was promoted to head the division of organic chemistry in 2017. He has authored over 150 scientific publications and has received several awards, including the Incentive Award in Synthetic Organic Chemistry, Japan (2006, 2010) and the Award for Young Investigators from the Japanese Peptide Society (2014).

Introduction PART 1: DESGIN OF CPPS Classification of cell-penetrating peptides Arginine-rich cell-penetrating peptides: recent advances of design and applications for intracellular delivery Cationic cell-penetrating peptides Amphipathic cell-penetrating peptides Hydrophobic cell-penetrating peptides Foldamers PART 2: MECHANISM OF CPPS Peptide Structure: Electrophysiological analysis, nuclear magnetic resonance analysis, and molecular dynamics simulation of direct penetration of cell-penetrating peptides through bilayer lipid membranes Cellular uptake mechanisms of arginine-rich cell-penetrating peptides Endosomal escape Pharmacokinetics of therapeutic and diagnostic agents conjugated with cell-penetrating peptides PART 3: DELIVERY TOOLS Drug delivery Peptide & protein delivery Cell-penetrating peptides for nucleic acid delivery PPMOs: a case study for cell-penetrating peptide application Cell penetrating peptide-conjugated polymer micelles for pDNA/siRNA delivery Lipid-based nanoparticles using cell-penetrating peptides PART 4: APPLICATIONS Cell-penetrating peptides as tools to facilitate oral delivery of biopharmaceuticals Intranasal delivery Clinical trials Applications in plants