This book is regarding brief study on Chlorophytum borivilianum belonging to family Liliaceae, popularly known, as safed musli is an important medicinal plant. Large-scale collections from the natural stands and destructive nature of harvesting, shy flowering with low seed formation, poor seed germination and low tuber multiplication ratio raise the concern about the possible extinction of the species. Thus Rapid micropropagation of Chlorophytum borivilianum through in vitro culture technique was studied by manipulation of hormone regime. At last genetic integrity between micropropagated plants and mother plants was studied using RAPD markers. All the bands produced remains monomorphic across all the micropropagated plants; no polymorphism was detected. Thus an efficient protocol for rapid mass propagation for Chlorophytum borivilianum was developed.