Chromatographic Methods de A. Braithwaite

Chromatographic Methods

A. Braithwaite

J.F. Smith

en Limba Engleză Paperback – 31 dec 1995

Offers an introduction to the general theory of chromatography, and a treatment of the principles and practice of the major techniques employed in the industrial and academic sectors. This book is suitable for students following the numerous Masters degrees in instrumental analysis.

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Cuprins

1 Introduction.- 1.1 Introduction to chromatography.- 1.2 History of chromatography.- 1.3 Classification of chromatographic methods.- References.- 2 Theoretical considerations.- 2.1 Introduction.- 2.2 Factors influencing retention.- 2.3 Retention and equilibrium in chromatography.- 2.4 Separating efficiency of a column.- 2.5 Band broadening processes.- 2.6 Resolution.- 2.7 Quantification in chromatography.- References.- 3 Planar chromatography.- 3.1 Thin layer chromatography.- 3.2 Paper chromatography.- 3.3 Electrophoresis.- 3.4 Capillary electrophoresis.- References.- 4 Liquid phase chromatography on open columns.- 4.1 Introduction.- 4.2 Practical aspects and considerations.- 4.3 Modes of chromatography.- 4.4 Adsorption chromatography.- 4.5 Partition column chromatography.- 4.6 Ion exchange chromatography.- 4.7 Inorganic ion exchangers.- 4.8 Gel ion exchangers.- 4.9 Gel chromatography.- 4.10 Affinity chromatography.- 4.11 Covalent chromatography.- 4.12 Chromatofocusing.- 4.13 Flash chromatography.- References.- 5 Gas chromatography.- 5.1 Introduction.- 5.2 Principles of gas chromatography.- 5.3 Resolution.- 5.4 Columns and stationary phase.- 5.5 Choice of stationary phase.- 5.6 Capillary column gas chromatography.- 5.7 Gas-solid chromatography.- 5.8 Gas chromatography instrumentation.- 5.9 Sampling techniques.- 5.10 Detectors for gas chromatography.- 5.11 Supercritical gas chromatography.- 5.12 Applications of gas chromatography.- References.- 6 High performance liquid chromatography.- 6.1 Introduction.- 6.2 Modes of chromatography.- 6.3 Overview of high performance liquid chromatography instrumentation.- 6.4 Theory.- 6.5 Detailed discussion of high performance liquid chromatography instrumentation.- 6.6 Column packings and stationary phases for liquid chromatography.-6.7 Adsorption chromatography.- 6.8 Liquid—liquid partition chromatography.- 6.9 Chemically bonded stationary phases for high performance liquid chromatography.- 6.10 Chiral chromatography.- 6.11 Ion exchange chromatography.- 6.12 Ion pairing.- 6.13 Size exclusion chromatography.- 6.14 Liquid chromatography method development.- 6.15 Quantitative analysis.- 6.16 Preparative liquid chromatography.- 6.17 Microcolumns in liquid chromatography.- 6.18 Applications.- References.- 7 Chromatography and spectroscopic techniques.- 7.1 Introduction.- 7.2 Chromatographic requirements.- 7.3 Chromatographic and mass spectrometry techniques.- 7.4 Gas chromatography interfacing techniques.- 7.5 High performance liquid chromatography interfacing.- 7.6 Chromatography and mass spectrometry data systems.- 7.7 Infrared spectrophotometry.- 7.8 Ultraviolet—visible spectrophotometry.- 7.9 Atomic spectroscopy.- References.- 8 Processing chromatographic data.- 8.1 Introduction.- 8.2 The chromatogram.- 8.3 Data collection and processing.- 8.4 Chromatography software.- 8.5 Method development and optimisation.- 8.6 Laboratory information management systems.- References.- 9 Model or practical experiments in chromatographic techniques.- 9.1 Introduction.- 9.2 List of experiments.- 9.3 Section A. Paper chromatography.- 9.4 Section B. Electrophoresis and related techniques.- 9.5 Section C. Thin layer chromatography.- 9.6 Section D. Column chromatography.- 9.7 Section E. Gas chromatography.- 9.8 Section F. High performance liquid chromatography.- 9.9 Section G. Capillary electrophoresis.- References.- 10 Illustrative problems in chromatography.- 10.1 Introduction.- 10.2 Plate theory and resolution.- 10.3 Van Deemter.- 10.4 Volatility and temperature in gas chromatography.- 10.5 Qualitativeanalysis.- 10.6 Quantitative analysis.- 10.7 Composite questions.- Appendix 1 Glossary of chromatographic terms.- Appendix 2 Table of chromatography symbols.