Plant transformation is a critical approach in the development of transgenic plants. The efficiency of transformation controls the transgenic events that may be established through a successful transformation procedure. The purpose to clone the ACTIN1 gene was to characterize its properties in cotton fiber growth in G.hirsutum in a respective TILLING project. The related data was collected from many sources up to 20 relating many bioinformatics databases or that particular project. Modifications were done at different places in the gene to get a good and a suitable clone with enhanced both genetical and physical which were TILLED introduced characters. On the bases of initial data of the project a database named COTTON TILLING DATABASE was established for the manipulation of data and for ease to the other people to retrieve the data from it. That facility quick searches and rapid retrieval of specific data. Queries to the database can be conducted through user friendly interface, and query result returned in the form of reports, which facilitates to save /or print the result. The data in cotton TILLING database is purely unique because it is not still a part of any data biomedical