The objectives of this study were to (1) determine if a reproducing population of crayfish belonging to the C. acuminatus complex occurs in Valley Creek within VFNHP; (2) conduct a comprehensive survey of Valley Creek within VFNHP and produce a list of all the crayfish species that occur there; and (3) determine the basic life history characteristics (e.g., size structure, sex ratio), reproductive status, and habitat preferences of the crayfish species that occur in Valley Creek within VFNHP.