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Cryo-Electron Microscopy in Structural Biology: From Structural Insights to Tomography and Drug Discovery de Krishnarao Appasani

Cryo-Electron Microscopy in Structural Biology: From Structural Insights to Tomography and Drug Discovery

Editat de Krishnarao Appasani
en Limba Engleză Hardback – 17 oct 2024
Cryo-electron microscopy, in combination with tomography, has emerged as a new technology for visualizing molecular structures at a resolution beyond even 1 Å. Using this technology has revealed the native molecular details of viruses, membranes, enzymes, ribosomes, and cells. This comprehensive volume brings together authoritative overviews of these methods from structural and biological perspectives. It is a must-have for researchers and graduate students, as well as those working in industry, primarily in the areas of biophysics, structural biology, crystallography, and genomics.
Key Features
• Focuses on the applications of cryo-EM to structural biology
• Documents the importance of cryo-EM/ET approaches in studying the structural determinants of cellular organelle and membrane protein biochemistry
• Reviews the applications of high-resolution structures of viruses
• Emphasizes structural insights of nuclear and gene machineries
• Includes a section focused entirely on the applications of cryo-EM/ET in drug discovery and therapeutic development
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Specificații

ISBN-13: 9781032352534
ISBN-10: 1032352531
Pagini: 486
Ilustrații: 302
Dimensiuni: 191 x 235 mm
Greutate: 1.05 kg
Ediția:1
Editura: CRC Press
Colecția CRC Press
Locul publicării:Boca Raton, United States

Public țintă

Postgraduate and Professional

Cuprins

SECTION I: Cryo-EM in Cell Biology - Structure Determination of Cellular Organelle1.Atomic-resolution protein structure determination by cryo-EM2.Sub-ångström cryo-EM Structure of a prion proto-fibrils3.Cryo-EM structure of native human uromodulin, a zona pellucida module polymer4.Chromatosome, telomere structure and genome maintenance using cryo-EM5.Cryo-EM of the dynamin super-family proteins6.Mito-ribosome structure using cryo-EMSECTION II: Technology Platform & Evolving Technologies1. Cryo-EM data in the cloud computing2.The silent revolution of cryo-EM and GPCR structures3.Development of world-first multi-gas plasma focused ion beam microscope4.3-D printed cell culture grid holders for improved cellular specimen preparation in cryo- electron microscopy5.Local resolution estimates of cryo-EM reconstruction6.A new cryo-EM system for electron 3D crystallographySECTION III: Cryo-EM in Virology - High-Resolution Structures of Viruses1.Cryo-Electron Microscopy structure of adeno-associated virus-DJ bound by a heparinoid pentasaccharide2.Cryo-EM and tomography of vesicular stomatitis viruses3.High-resolution structures of HIV-1 gag cleavage mutants4.Cryo-electron microscopy of the T7 bacteriophage portal and fiber-less tail complex5.SARS-Co-2 spike protein structure determinationSECTION IV: Cryo EM in Membrane Biology – Probing the structures of Membrane Proteins1.Structure and activity of lipid bilayer within a membrane-protein transporter2.Cryo-EM structure of human Rhodopsin bound to an inhibitory G protein3. Cryo-EM structure of the acetylcholine receptor that cause congenital myasthenic syndromes4.Structure of the mitochondrial F-ATPase as determined by electron cryo-microscopy5.Cryo-EM structure of cardiac amyloid fibrils from an immunoglobulin light chain AL amyloidosis patientSECTION V: Cryo-EM in Molecular Biology - Structural Insights of Nuclear and Gene Machineries1.Structural information from cryo-EM studies on multicomponent molecular assemblies such as those found in DNA-double-strand-break repair2. Cryo-EM structure of the DNA-PK holoenzyme3. Cryo-EM reconstruction at 4.0 Å of human Polymerase III4.Cryo-electron microscopy structures of UV-DDB bound to nucleosome5. Cryo-electron microscopy of the chromatin fiber6. Time-resolved cryo-EM studies in the ribosome dynamicsSECTION VI: Cryo-Tomography and Emerging Technologies1.Cryo-Electron Tomography in membrane protein structural research2.Cryo-tomography of membrane transporters involved in neurological psychiatric disease3.Enveloped viruses and coated vesicles - electron cryo-microscopy and tomography 4.Electron cryo-tomography in the study of autophagy or bacterial secretion systems5.DNA Origami sign posts for identifying proteins on cell membranes by cryo-tomography6.3-D deconvolution processing for STEM cryo-tomographySECTION VII: Applications of Cryo-EM/ET in Drug Discovery1.Cryo-EM to enable research and drug discovery of CD20 in complex with the therapeutic monoclonal antibody rituximab2. Fragment-based drug discovery using cryo-EM3.CryoEM approaches to study allosteric regulation of GTP cyclohydrolase I4.Cryo-EM in drug discovery5.Structural insights of an advanced vaccine candidate as derived from SARS-CoV-2 spike protein6. Unleashing the power of Cryo-EMEpilogue

Notă biografică

Krishnarao Appasani trained as a molecular cell biologist, earning Masters and Doctoral degrees in Biochemistry and Molecular biology. He accepted a Lectureship at Boston University and taught cell and molecular biology courses to undergraduates and graduate students. He was a post-doc in the laboratory of Nobel Laureate, the late Prof. H. Gobind Khorana at the Massachusetts Institute of Technology. Subsequently, he joined the faculty of Harvard Medical School and directed the Thoracic Oncology Laboratory at the Brigham &Women’s Hospital and the Dana-Farber Cancer Institute in Boston. During this time he completed an MBA at Bryant University, Smithfield, RI, and then moved to Perkin Elmer and directed the Microarrays group. Dr Appasani has published about 60 research papers and abstracts in international journals, especially on Gene Expression. He was awarded the Outstanding Investigator Award by the American Federation for Medical Research, Washington D.C., competed globally for the Ranbaxy Research Medal, and was honored with the 1997-Ranbaxy Research Award in the field of Applied Medical Sciences. At Perkin Elmer, Dr Appasani organized a global Biomics Seminar series to educate scientists and to initiate and develop collaborations with researchers in academia and other industries. For about 15 years he ran a multi-million-dollar knowledge management enterprise and served as an international authority in bridging Academia and Industry by fostering inter-disciplinary and translational science. In 2016 Dr Appasani was inducted as a Life Fellow of the Royal Society of Biology, England, for his global efforts in promoting science education.

Descriere

Cryo-Electron Microscopy, in combination with Tomography, has emerged as a new technology for visualizing molecular structures revealing the native molecular details of membranes, viruses, and cells. This comprehensive, volume brings together authoritative overviews of these methods from structural and biological perspective.