This study examines the role of culture in the adoption of measures and strategies for the prevention of HIV/ AIDS among girls and women in Western Kenya. It focuses on levels of awareness of HIV/AIDS prevention measures and patterns of adoption of five the measures that are currently being promoted in Kenya:the use of condoms; screening for HIV at Voluntary Counselling and Testing (VCT) Centres; prevention of mother-to-child transmission (PMTCT); the use of post-exposure prophylaxis (PEP); and the prompt treatment of sexually transmitted infections (STIs). This research is focused on examining the cultural context of HIV/AIDS research and programmes in Western Kenya and aims to reveal how limited has been the critical analysis of culture as a determinant of adoption of these measures despite the stated prioritization of culture as a key factor to consider.