The organism responsible for a Diabetic foot is clostridium species in the west 1], hence, we need to know the most common organism involved in DF in our setup. Our research project sheds light on the prevalence of most common organism responsible for diabetic infection and to find out their sensitivity to antimicrobial agents to prevent amputation and sepsis by the administration of empirical treatment. The objective of this study was to analyze the bacterial isolates of all patients admitted to LADY READING HOSPITAL, KHYBER TEACHING HOSPITAL and HAYATABAD MEDICAL COMPLEX, presented with diabetic foot infection in Wagner grade 2-5.