Aim of the study to improve the genetic variability of a medicinal plant Trigonella foenum-garecum L. through the mutation breeding. After the treatment of mutagens (MMS and SA) it concluded that lower and moderate concentrations of chemical mutagens proved to be efficient in increasing the genetic variability for yield oriented selection in fenugreek and the isolated variants possessed desirable characters associated with higher yield, may be evaluated in future generations for promising performance. Thus the genetic variability induced by mutagenesis may be effectively exploited for the improvement of fenugreek.