Infertility is a medical condition or clinical term for couples who do not have the ability to achieve pregnancy from unprotected sexual contact for a period of 12 months. vitamin D is considered one of the important causes of many diseases, as the current study evaluated the levels of this vitamin with the anti-Mullerian hormone (AMH) in women with infertility, both primary and secondary types, in addition to that nanotechnology is one of the modern technologies that have taken a wide field and have many applications In many fields due to its chemical, optical and engineering properties. Therefore, researchers in chemistry, biology, medicine and other fields were interested in the sciences of nanomaterials, So, the study aimed to find out the role and effect of nanoparticles of metals (Se NPs) and metal oxides (CuO NPs) on levels of vitamin D and AMH in women with primary and secondary infertility.