The book on Culture Media, Stains and Reagents was compiled to facilitate the students, teachers and researchers of Microbiology for their laboratory work. This book is divided into chapters on Media for bacteria, fungi, Actinomycetes, algae, stains, reagents and buffers for easy reference of the user. The material of this book is the outcome of three decades of research experience of the author, both as a Scientist and Research Guide for University Scholars. Several standard source books were referred for the preparation of this manuscript. A treatise on microbiological media is the efforts of my student's research and their agonized frustration to locate the media composition and related information needed to culture specific microorganism or to stain them or to carry out biochemical tests for identifying the isolates. My research experience and my research scholars are the stimulus for writing this comprehensive reference book on the culture media. They are listed alphabetically with composition, instructions for preparations and uses. I acknowledge the moral support of my Guru Dr. B.D. Kaushik, students, colleagues and family members.My thanks to LAP publishers