Make the most of your lab time with this interactive manual by text author Shawn O. Farrell and co-author Lynn E. Taylor. You?ll find a selection of classroom-tested experiments designed to be completed during a normal laboratory period. Benefits: * NEW In Experiment 12, students will clone the barracuda LDH gene into an expression vector, making a HIS-Tagged fusion protein that can be purified by affinity chromatography. Students will create a new vector for doing expression that makes the ligation of DNA easier and more efficient while learning about expanded methods for purification of HIS-Tagged Lactate Dehydrogenase via Affinity Chromatography. * NEW The Second Edition includes expanded problem sets for further biochemistry practice and study. * All experiments have been classroom tested through multiple semesters and can be completed in a normal lab period. * Alternate versions of experiments allow for easy use in labs which meet only once a week or multiple times in a week. * "Tip" boxes throughout text give students pointers on how to perform the labs. * "Essential Information" boxes highlight pertinent information. * "References and Further Reading" sections are at the end of each chapter. * An additional "Problem Set" appears in each chapter.