Erythromycin producing bacteria Saccharopolyspora erythraea strain MTCC No. 1103 was purchased from MTCC, Chandigarh. Sporulation of this bacteria was done on Oat meal Agar medium at a temperature of about 30˚C. Optimization of the fermentation conditions was performed with different parameter,like temperature, pH, time etc. Fermentative production of erythromycin gave best results with a temperature of 30˚C at a pH of 7.5 in 5 days. Further research works are going on process optimization with shake flask & bioreactors.