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Flow Cytometry Today: Everything You Need to Know about Flow Cytometry

Autor Claudio Ortolani
en Limba Engleză Paperback – 17 noi 2022
This book covers all the technical aspects of flow cytometry needed to set-up the instrument, solve problems encountered in daily work, or necessary for exam preparation. It provides the reader with an in-depth look at the device and its applications.
Each component and its function is described in an easy-to-understand manner, giving the reader a sound basic knowledge of this instrument. The practical examples given, simplify and enhance the learning process. This book is a unique resource of knowledge for biomedical engineers and biotechnologists, flow cytometry operators, laboratory technicians and biomedical researchers, both biologists as well as medical doctors, and can also be a helpful tool for companies and manufacturers.

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Specificații

ISBN-13: 9783031108358
ISBN-10: 3031108353
Pagini: 544
Ilustrații: XXIX, 544 p. 1 illus.
Dimensiuni: 155 x 235 mm
Greutate: 1.07 kg
Ediția:1st ed. 2022
Editura: Springer International Publishing
Colecția Springer
Locul publicării:Cham, Switzerland

Cuprins

Chapter 1. General Principles.- Chapter 2. Signals – Scattering.- Chapter 3. Signals - Fluorescence, Phosphorescence, Impedance, Extinction.- Chapter 4. Fluidics.- Chapter 5. Light Sources.- Chapter 6. Optical Benches.- Chapter 7. Detectors and Electronics.- Chapter 8. Signal Analysis.- Chapter 9. The Cytometric File.- Chapter 10. Data Transformation.- Chapter 11. Data Representation.- Chapter 12. Data Analysis.- Chapter 13. Standards, Setup, Calibration, and Control Techniques.- Chapter 14. Fluorochromes – Overview.- Chapter 15. Fluorochromes Suitable for Antibody Conjugation.- Chapter 16. Fluorochromes That Bind Nucleic Acids.- Chapter 17. Fluorochromes for the Study of the Cell Features.- Chapter 18. Fluorescent Proteins.- Chapter 19. Spillover and Compensation.- Chapter 20. Artifacts.- Chapter 21. Cell Sorting.- Chapter 22. Non-Conventional Flow Cytometry.- Chapter 23. Statistics: A Cytometric Point of View.

Notă biografică

Dr. Claudio Ortolani was a senior consultant (now retired) in Clinical Pathology at the Hospital Dell’Angelo, Venice, Italy.

Textul de pe ultima copertă

This book covers all the technical aspects of flow cytometry needed to set-up the instrument, solve problems encountered in daily work, or necessary for exam preparation. It provides the reader with an in-depth look at the device and its applications.
 
Each component and its function is described in an easy-to-understand manner, giving the reader a sound basic knowledge of this instrument. The practical examples given, simplify and enhance the learning process.  
This book is a unique resource of knowledge for biomedical engineers and biotechnologists, flow cytometry operators, laboratory technicians and biomedical researchers, both biologists as well as medical doctors, and can also be a helpful tool for companies and manufacturers.


Caracteristici

Explains the technical details of a flow cytometer Prepares the reader for solving potential problems in daily laboratory work Covers all aspects of flow cytometric measurements