Forensic Genetic Approaches for Identification of Human Skeletal Remains: Challenges, Best Practices, and Emerging Technologies
Editat de Angie Ambersen Limba Engleză Hardback – 15 noi 2022
Unidentified human remains are often associated with tragic events, such as fires, terrorist attacks, natural disasters, war conflicts, genocide, airline crashes, homicide, and human rights violations under oppressive totalitarian regimes. In these situations, extensive damage to soft tissues often precludes the use of such biological samples in the identification process. In contrast, bone material is the most resilient, viable sample type for DNA testing. DNA recovered from bone often is degraded and in low quantities due to the effects of human decomposition, environmental exposure, and the passage of time. The complexities of bone microstructure and its rigid nature make skeletal remains one of the most challenging sample types for DNA testing.
- Provides best practices on processing bone samples for DNA testing
- Presents detailed coverage of proper facilities design for skeletal remains processing, selection of optimal skeletal elements for DNA recovery, specialized equipment needed, preparation and cleaning of bone samples for DNA extraction, and more
- Highlights complementary disciplines often used in concert with genetic data to make positive identifications, such as forensic anthropology, forensic odontology, and forensic art/sculpting
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Specificații
ISBN-13: 9780128157664
ISBN-10: 0128157666
Pagini: 626
Dimensiuni: 191 x 235 x 31 mm
Greutate: 0.96 kg
Editura: ELSEVIER SCIENCE
ISBN-10: 0128157666
Pagini: 626
Dimensiuni: 191 x 235 x 31 mm
Greutate: 0.96 kg
Editura: ELSEVIER SCIENCE
Public țintă
Primary: Forensic Genetics Practitioners; Forensic Pathologists; Disaster Victim Identification (DVI) Workers; Upper division undergraduate and graduate students in forensic genetics.Secondary: Forensic Anthropologists, Bioarchaeologists, Attorneys and Legal Practitioners, Museum Curators, and Historical Societies interested in current approaches and capabilities of DNA testing on human skeletal remains.Cuprins
1. Missing persons and unidentified human remains: The world’s silent mass disaster 2. Challenges in forensic genetic investigations of decomposed or skeletonized human remains: Environmental exposure, DNA degradation, inhibitors, and low copy number (LCN)
I. Guidelines and best practices for handling and processing human skeletal remains for genetic studies 3. Facilities design and workflow considerations for processing unidentified human skeletal remains 4. Location, recovery, and excavation of human remains for forensic testing 5. Skeletal microstructure, bone diagenesis, optimal sample selection, and pre-processing preparation techniques for DNA testing 6. DNA extraction methods for human skeletal remains 7. Quantitative and qualitative assessment of DNA recovered from human skeletal remains
II. Types of DNA markers and applications for identification 8. Autosomal short tandem repeat (STR) profiling of human 9. Y-chromosome analysis for unidentified human remains (UHR) investigations 10. Mitochondrial DNA and its use in the forensic analysis of skeletal material 11. X-chromosome short tandem repeats (X-STRs): Applications for human remains identification 12. Single nucleotide polymorphisms (SNPs): Ancestry-, phenotype-, and identity-informative SNPs 13. Diallelic Markers: INDELs and INNULs
III. Traditional platforms, alternative strategies, and emerging technologies for DNA analysis of human skeletal remains 14. Genotyping and sequencing of DNA recovered from human skeletal remains using capillary electrophoresis (CE) 15. Rapid DNA identification of human skeletal remains 16. Emerging technologies for DNA analysis of challenged samples
IV. Analysis of genetic data recovered from skeletonized human remains 17. Best practices in the development and effective use of a forensic DNA database for identification of missing persons and unidentified human remains 18. Software and database functionality for direct identification and kinship analysis: The Mass Fatality Identification System (M-FISys) 19. Bioinformatic tools for interrogating DNA recovered from human skeletal remains 20. The emerging discipline of forensic genetic genealogy
V. Complementary and multidisciplinary approaches to assist in identification of unidentified human skeletal remains 21. Forensic anthropology in a DNA world: How anthropological methods complement DNA-based identification of human remains 22. Generation of a personal chemical profile from skeletonized human remains 23. Forensic odontology: Historical perspectives and current applications for identification of human remains 24. Forensic facial reconstruction of skeletonized and highly decomposed human remains
I. Guidelines and best practices for handling and processing human skeletal remains for genetic studies 3. Facilities design and workflow considerations for processing unidentified human skeletal remains 4. Location, recovery, and excavation of human remains for forensic testing 5. Skeletal microstructure, bone diagenesis, optimal sample selection, and pre-processing preparation techniques for DNA testing 6. DNA extraction methods for human skeletal remains 7. Quantitative and qualitative assessment of DNA recovered from human skeletal remains
II. Types of DNA markers and applications for identification 8. Autosomal short tandem repeat (STR) profiling of human 9. Y-chromosome analysis for unidentified human remains (UHR) investigations 10. Mitochondrial DNA and its use in the forensic analysis of skeletal material 11. X-chromosome short tandem repeats (X-STRs): Applications for human remains identification 12. Single nucleotide polymorphisms (SNPs): Ancestry-, phenotype-, and identity-informative SNPs 13. Diallelic Markers: INDELs and INNULs
III. Traditional platforms, alternative strategies, and emerging technologies for DNA analysis of human skeletal remains 14. Genotyping and sequencing of DNA recovered from human skeletal remains using capillary electrophoresis (CE) 15. Rapid DNA identification of human skeletal remains 16. Emerging technologies for DNA analysis of challenged samples
IV. Analysis of genetic data recovered from skeletonized human remains 17. Best practices in the development and effective use of a forensic DNA database for identification of missing persons and unidentified human remains 18. Software and database functionality for direct identification and kinship analysis: The Mass Fatality Identification System (M-FISys) 19. Bioinformatic tools for interrogating DNA recovered from human skeletal remains 20. The emerging discipline of forensic genetic genealogy
V. Complementary and multidisciplinary approaches to assist in identification of unidentified human skeletal remains 21. Forensic anthropology in a DNA world: How anthropological methods complement DNA-based identification of human remains 22. Generation of a personal chemical profile from skeletonized human remains 23. Forensic odontology: Historical perspectives and current applications for identification of human remains 24. Forensic facial reconstruction of skeletonized and highly decomposed human remains