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Gene Biotechnology de William Wu

Gene Biotechnology

Autor William Wu, Helen H. Zhang, Michael J. Welsh, Peter B. Kaufman
en Limba Engleză Hardback – 18 mai 2011
Covering state-of-the-art technologies and a broad range of practical applications, the Third Edition of Gene Biotechnology presents tools that researchers and students need to understand and apply today's biotechnology techniques. Many of the currently available books in molecular biology contain only protocol recipes, failing to explain the principles and concepts behind the methods outlined or to inform the reader of possible pitfalls in the methods described. Filling these gaps, this book:
  • Discusses a wide variety of approaches, from very basic methods to the latest, most sophisticated technologies
  • Contains clearly detailed, step-by-step protocols with helpful troubleshooting tips
  • Addresses the needs of researchers in academic and commercial environments
  • Guides graduate students in designing, implementing, and evaluating experimental projects.
Each chapter covers the principles underlying methods and techniques, and includes step-by-step descriptions of each protocol, notes, tips, and a troubleshooting guide. The book includes sections on how to write a research paper for publication in English-language journals, how to protect research discoveries and inventions via patents, and practical methods of bio-calculation.
Written by a team of internationally recognized scientists, Gene Biotechnology presents protocols as well as clear and simple explanations of the key principles and concepts behind the methods. It is a single, logically organized source for the most important new methodologies. This unique resource provides the tools to help ensure success in contemporary molecular and cellular biology research.
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Specificații

ISBN-13: 9781439848302
ISBN-10: 1439848300
Pagini: 576
Ilustrații: 140 b/w images and 1 table
Dimensiuni: 156 x 234 x 33 mm
Greutate: 0.93 kg
Ediția:Revizuită
Editura: CRC Press
Colecția CRC Press

Public țintă

Professional

Cuprins

Strategies for Novel Research Projects and Research Grant Funding. Rapid Isolation of Specific cDNA or Gene by PCR. Construction and Screening of Subtracted and Complete Expression cDNA Libraries. Subcloning of Genes or DNA Fragments. Non-isotopic and Isotopic DNA or RNA Sequencing. Information Superhighway and Computer Databases of Nucleic Acids & Proteins. Characterization of DNA or Genes by Southern Blot Hybridization. Gene Over-expression by Sense RNA in Mammalian System. Gene Under-expression by Antisense DNA and RNA Strategies. Analysis of Gene Expression at the Functional Genomic Level Using Northern blotting and/or PCR. Analysis of Gene Expression at the Proteomic Level via Western Blotting. Analysis of Protein Expression Using Immuno-fluorescence Localization. Analysis of Cellular DNA or Abundance mRNA by Radioactivity In Situ Hybridization (RISH). Localization of DNA or Abundance mRNA by Fluorescence In Situ Hybridization (FISH). In Situ PCR Hybridization of Low Copy Gene and In Situ RT-PCR. Detection of Low Abundance mRNA. Isolation and Characterization of Genes from Genomic DNA Libraries. Mouse Stem Cells as a Model Mammalian Cell line for Gene Expression. New Strategies for Gene Knockout. Large-scale Expression and Purification of Recombinant Proteins. Quantitative Analysis of Functional Genome by Real-Time RT –PCR. High-throughput Analysis of Gene Expression by DNA Microarrays (Gene Chips). Construction and Screening of Human Antibody Libraries Using Phage Display Technology. Down-regulation of Gene Expression in Mammalian Systems via SiRNA Technology. New Techniques for Identification of Protein Binding and Proteomic Interactions.

Notă biografică

William Wu, Ph.D., has extensive experience in both academic and industrial settings. He held various positions, including Research Assistant Professor at the University of Michigan Medical School, Ann Arbor, Michigan, USA, and Professor at the Hunan Nonnal University, Hunan, the People's Republic of China, Senior Scientist, Team-Leader and Chief Technology Officer (CTO) in biomedical and pharmaceutical companies.
Helen H. Zhang, MS., is Senior Research Associate at the University of Michigan Medical School, Ann Arbor, Michigan, USA. In 1996, she received her MS degree in Molecular and Cellular Biology at the Eastern Michigan University, Ypsilanti, Michigan, USA.
Michael J. Welsh, Ph.D., is Professor of Cell Biology in the Department of Cell and Developmental Biology at the University of Michigan Medical School, Ann Arbor, Michigan, USA. He is also a Professor of Toxicology in the School of Public Health at the University of Michigan, Ann Arbor.
Peter B. Kanfman, Ph.D., is Professor of Biology in the Plant Cellular and Molecular Biology Program in the Department of Biology and a member of the faculty of the Bioengineering Program at the University of Michigan.

Recenzii

I can recommend this book to any molecular biologist without hesitation and would not be surprised if soon it establishes itself as one of the definitive guides to laboratory practice.
Annals of Botany, 2005

Descriere

The third edition of this popular reference covers a variety of techniques related to gene manipulation, including DNA isolation, preparation, screening, and analysis. Topics range from very basic methods to current and sophisticated technologies, including methodologies created and tested by the authors. The authors offer detailed, step-by-step explanations of protocols and helpful troubleshooting guides. The book also discusses approaches to grant funding and SiRNA technology. This edition features new techniques in every chapter, as well as several new chapters.