As a part of biochemistry, gene biotechnology amalgamates the elements of genetics and biotechnology to understand and experiment with genes in order to develop better genetically modified and cloned organisms. Gene isolation and gene cloning is also a part of gene biotechnology. This book is designed to provide in-depth information about the various concepts, theories, techniques and methods related to gene biotechnology. It presents chapters which deal with the most important topics in this field and provide detailed information about its applications. As this field is emerging at a rapid pace, the contents of this textbook will help the readers understand the modern concepts of the subject.