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Gene Therapy Protocols: Methods in Molecular Medicine, cartea 07

Editat de Paul D. Robbins
en Limba Engleză Hardback – 31 ian 2010
Thirty protocols by leading researchers describe in detail all the essential molecular methods for working with gene transfer systems, along with the methods for gene transfer to specific tissue types either in vivo or ex vivo. The easily reproducible methods range from those for specific viral and nonviral for both genetic and acquired diseases, to those concerned with gene delivery to particular tissues. Methods for applying specific therapeutic systems, such as ribozymes and tumor supressor genes for the treatment of AIDS and cancer, are also included in this authoritative collection.
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Specificații

ISBN-13: 9780896034846
ISBN-10: 0896034844
Pagini: 452
Dimensiuni: 161 x 237 x 35 mm
Greutate: 0.88 kg
Editura: Humana Press Inc.
Seria Methods in Molecular Medicine

Locul publicării:Totowa, NJ, United States

Public țintă

Professional/practitioner

Cuprins

Methods for Gene Transfer Using DNA-Adenovirus Conjugates, David T. Curiel. Methods for the Construction and Propagation of Recombinant Adeno-Associated Virus Vectors, Jeffrey S. Bartlett and Richard J. Samulski. Generation of High-Titer, Helper-Free Retroviruses by Transient Transfection, Warren S. Pear, Martin L. Scott, and Garry P. Nolan. Methods for the Construction of Retroviral Vectors and the Generation of High-Titer Producers, Isabelle Rivière and Michel Sadelain. Development of Replication-Defective Herpes Simplex Virus Vectors, David Krisky, Peggy Marconi, William F. Goins, and Joseph C. Glorioso. Methods for the Use of Poliovirus Vectors for Gene Delivery, Casey D. Morrow, David C. Ansardi, and Donna C. Porter. Methods for the Construction of Human Papillomavirus Vectors, Saleem A. Khan and Francis M. Sverdrup. Methods for Liposome-Mediated Gene Transfer to the Arterial Wall, Elizabeth G. Nabel, Zhi-yong Yang, Hong San, Dianne P. Carr, and Gary J. Nabel. Methods for Targeted Gene Transfer to Liver Using DNA-Protein Complexes, Mark A. Findeis, Catherine H. Wu, and George Y. Wu. Methods for the Use of Retroviral Vectors for Transfer of the CFTR Gene to Airway Epithelium, John C. Olsen, Larry G. Johnson, and James R. Yankaskas. Methods for Adenovirus-Mediated Gene Transfer to Airway Epithelium, John F. Engelhardt. Methods for Retrovirus-Mediated Gene Transfer to Fetal Lung, Bruce R. Pitt, Margaret A. Schwarz, and Richard D. Bland. In Situ Retrovirus-Mediated Gene Transfer into the Liver, Nicolas Ferry, Sophie Branchereau, Jean-Michel Heard, and Olivier Danos. Methods for Delivery of Genes to Hepatocytes In Vivo Using Recombinant Adenovirus Vectors, Darlene Barr and Mark A. Kay. Methods for Producing High Titer, Pantropic Retroviral Vectors for Gene Transfer into Leukemic T-Cells, Jane C. Burns, Jiing-Kuan Yee, and Alice L. Yu. Methods for Efficient Retrovirus-Mediated Gene Transfer to Mouse Hematopoietic Stem Cells, John W. Belmont and Roland Jurecic. Methods for Retrovirus-Mediated Gene Transfer into Primary T-Lymphocytes, Michel Sadelain. Methods for Retrovirus-Mediated Gene Transfer to CD34+ Enriched Cells, Alfred B. Bahnson, Maya Nimgaonkar, Edward D. Ball, and John A. Barranger. Methods for the Use of Genetically Modified Keratinocytes in Gene Therapy, Sabine A. Eming and Jeffrey R. Morgan. Methods for Particle-Mediated Gene Transfer into Skin, Ning-Sun Yang, Dennis E. McCabe, and William F. Swain. Methods for Liposome-Mediated Gene Transfer to Tumor Cells In Vivo, Gary J. Nabel, Zhi-yong Yang, and Elizabeth G. Nabel. Methods for Retrovirus-Mediated Gene Transfer to Tumor Cells, Elizabeth M. Jaffee. Cationic Liposome-Mediated Gene Transfer to Tumor Cells In Vitro and In Vivo, Kyonghee Son, Frank Sorgi, Xiang Gao, and Leaf Huang. Methods for the Use of Cytokine Gene-Modified Tumor Cells in Immunotherapy of Cancer, Zhihai Qin and Thomas Blankenstein. Methods for Generation of Genetically Modified Fibroblasts for Immunotherapy of Cancer, Elaine M. Elder, Michael T. Lotze, and Theresa L. Whiteside. Methods for Gene Transfer to Synovium, Richard Kang, Paul D. Robbins, and Christopher H. Evans. Methods for Adenovirus-Mediated Gene Transfer to Synovium In Vivo, Blake J. Roessler. Methods for the Use of Stromal Cells for Therapeutic Gene Therapy, Joel S. Greenberger. Suppression of the Human Carcinoma Phenotype by an Antioncogene Ribozyme, Toshiya Shitara and Kevin J. Scanlon. Methods for Cancer Gene Therapy Using Tumor Suppressor Genes, Wei-Wei Zhang and Jack A. Roth. Index.


Recenzii

Such a compilation is of great interest fro researchers who get interested in the protocols utilized in gene transfer and in thier applications. Furthermore, for researchers already involved in gene therapy, this book is a very good reference with a "note section" containing many details and comments, useful to improve current protocols.-ACTA Neurologica Belgica

Descriere

Thirty protocols by leading researchers describe in detail all the essential molecular methods for working with gene transfer systems, along with the methods for gene transfer to specific tissue types either in vivo or ex vivo. The easily reproducible methods range from those for specific viral and nonviral for both genetic and acquired diseases, to those concerned with gene delivery to particular tissues. Methods for applying specific therapeutic systems, such as ribozymes and tumor supressor genes for the treatment of AIDS and cancer, are also included in this authoritative collection.