Candida albicans biofilm formation is a poorlyunderstood, complex biological process withsignificant consequences for human health. In thisthesis, I describe the progress I have made inelucidating the genetic mechanisms of Candidaalbicans biofilm formation using a large-scale genedisruption strategy. The Bcr1-dependent adherenceregulatory pathway that I discovered is pivotal toour understanding of C. albicans biofilm formation. Without the transcription factor Bcr1, named forBiofilm and Cell-wall Regulator, C. albicans cellsare unable to form biofilms, a process that isextremely important to its lifestyle andpathogenicity. Expression analysis indicates thatBcr1 activates cell surface protein and adhesingenes, whose gene products could be used to developnew anti-fungal therapies.