These proceedings provide a synopsis of the Third Workshop on Genetics of Bark Beetles and Association Microorganisms, which was held May 20-2, 2006 in Asheville, NC. Twenty- five participants from five countries attended the meeting. The proceedings are structured into four parts: Phylogenetics of Bark Beetles, Population Genetics of Bark Beetles, Bark Beetle Gene Structure and Function, and Genetics of Symbionts, Natural Enemies, and Hosts. The abstracts give a snapshot of our current understanding of the genetics of bark beetles and associated microorganisms.