β-glucan was extracted from different sources such as barley, yeast, and plant extract (garlic and ginger). The effect of β-glucan was examined on the total cholesterol, high density lipoproteins (HDL) and low density lipoproteins (LDL) level in mice. β-glucan extracted from barley lowered the total cholesterol from 180 mg/dl to 125 mg/dl, LDL from 30 to 27 and it showed no effect on HDL level. Yeast extracted β-glucan reduces total cholesterol from 180 mg/dl to 40 mg/dl and LDL value from 30 mg/dl to 08 mg/dl; however it showed no effect on HDL value. Plant extract (garlic and ginger) showed the lowering of total cholesterol from 180 mg/dl to 171 mg/dl, and LDL value from 30 mg/dl to 10 mg/dl; however plant extract showed the improvement of HDL value from 40 mg/dl to 125 mg/dl.