Handbook of Phycological Methods de Johan A. Hellebust

Handbook of Phycological Methods: Physiological and Biological Methods

Johan A. Hellebust

James S. Craigie

This 1979 volume of the Handbook of Phycological Methods was the first compilation of biochemical and physiological procedures selected specifically for the experimental phycologist. Algae present unique problems to the researcher because of their diverse structure and composition, which differ significantly from those of other commonly used organisms. The book contains practical examples of processes such as photosynthesis, respiration and the transport and accumulation of ions presented by contributors who have applied these these techniques to algae. The methods are described in sufficient detail so that they may be used by technicians, graduate and undergraduate students, and non-specialists. Beginners and experts alike will appreciate the continuing value of this book.

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Contributors; Editors' preface; Introduction; Part I. Isolation of Organelles and Membranes: 1. Chloroplasts from Euglena gracilis C. A. Price; 2. Nuclei and chromatin from Euglena gracilis D. E. Buetow; 3. Mitochondria from Chlorella Neil G. Grant; 4. Microbodies from Chlorogonium and Spirogyra Helmut Stabenau; 5. Isolation and characterization of diatom membranes Cornelius W. Sullivan; Part II. Analysis of Chemical Constituents: 6. Chlorophylls and carotenoids Arne Jensen; 7. Algal biliproteins Harold W. Siegelman and J. Helen Kycia; 8. Nucleic acids Rose Ann Cattolico; 9. Protein determination by dye binding Gary Kochert; 10. Carbohydrate determination by the phenol-sulfaric acid method Gary Kochert; 11. Glycerolipids and fatty acids of algae J. P. Williams; 12. Carrageenans and agars J. S. Craigie and C. Leigh; 13. β-1,3-Glucans in diatoms and brown seaweeds Sverre Myklestad; 14. Alginic Acid Bjørn Larsen; 15. Phenolic compounds in brown and red algae Mark A. Ragan and J. S. Craigie; 16. Brown seaweeds: analysis of ash, fiber, iodine and mannitol Bjørn Larsen; 17. Quantitation of the macromolecular components of microalgae Gary Kochert; 18. ATP, ADP, and AMP determinations in water samples and algal cultures David M. Karl and Osmund Holm-Hansen; Part III. Enzymes: 19. Ribulose-bis-phosphate carboxylase in Chlamydomonas S. S. Badour; 20. Nitrate reductase in marine phytoplankton Richard W. Eppley; 21. Threonine deaminase in marine microalgae N. J. Antia and R. S. Kripps; 22. Phosphoenolpyruvate carboxylse of blue-green algae Brian Colman; 23. Aldolases of multicellular marine algae Kazutosi Nisizawa; 24. The glucosyltranserases of red algae Jerome F. Frederick; 25. Anion-activated adenosine triphosphates Paul G. Falkowski; 26. Extracellular acid phosphatase of Ocheomonas sanica N. J. Patni and S. Aaronson; Part IV. Physiological and Biochemical Processes: 27. Determination of photosynthetic rates and 14C photoassimilatory products of brown seaweeds Bruno P. Kremer; 28. Polargraphic measurements of respiration following light-dark transitions Alan D. Jassby; 29. Hill reaction and photophosphorylation with chloroplast perparations from Chlamydomonas reinhardi Stephen Lien; 30. Respiratory assimilations of 14C-labelled substrates by a microalga Keith E. Cooksey; 31. Respiratory processses in mitochondria Neil G. Grant; 32. Determination of protein synthesis during synchronous growth Dennis J. O'Kane and Raymond F. Jones; 33. Nucleic acid synthesis Rose Ann Cattolico; 34. Nitrogen fixation Timothy H. Mague; 35. Uptake of organic substrates Johan A. Hellebust and Yi-Hung Lin; 36. Release of organic substances C. Nalewajko; Part V. Nutrients: 37. Nitrate uptake Richard W. Eppley; 38. Phosphate uptake F. P. Healey; 39. Sulfate uptake Monica Lik-Shing Tsang, Robert C. Hodson and Jerome A. Schiff; 40. Uptake of silicic acid Farooq Azam and Sallie W. Chisholm; 41. Uptake of micronutrients and toxic metals Anthony G. Davies; Part VI. Ion Content and Transport: 42. Measurement of ionic content and fluxes in microalgae J. Barber; 43. Ion transport and ion-stimulated adenosine triphosphatases Cornelius W. Sullivan; Part VII. Inhibitors: 44. Inhibitors used in studies of algal metabolism S. S. Badour; Part VIII. Appendix; Part IX. Indexes.