•Seroprevalence of HCV among patients ,blood volunteers and medical staff. •Prevalence Detection of HCV according age, gender and geography. •ELISA technique used for detection of HCV antibodies titer. •Detection of viral load in blood of patients and prove the serodiagnosis of hepatitis by Real Time PCR. •Genotyping of HCV through Reverse Transcription Polymerase Chain Reaction (RT-PCR) (Extraction of RNA ,Reverse transcription for cDNA ,Amplification and Detection.).