The Florida 5th Infantry Regiment completed its organization at Tallahassee, Florida, during the spring of 1862. The regiment was ordered to Virginia with over 1,000 men where it served in General Pryor's, E.A. Perry's, and Finegan's Brigade, Army of Northern Virginia. The regiment fought with the army from Second Manassas to Cold Harbor, then was active in the Petersburg trenches south of the James River and the Appomattox Campaign. Companies of the Florida 5th Infantry Regiment Companies that made up the Florida 5th Infantry Regiment were from the counties of Santa Rosa, Baker, Polk, Calhoun, Okaloosa, Wakulla, Madison, and Liberty.