Diabetes mellitus (DM) is a world-wide endemic with increasing prevalence in both developing and developed countries in a common cause of disability and death all over the world .The outburst of diabetes in the word is mainly due to type 2 diabetes about (90 - 95%). Its specific etiology is not yet known its frequency varies in different racial and ethnic subgroups and is often associated with a strong familial, likely genetic predisposition. It was denoted that type2 DM was associated with certain HLA class II alleles were analyzed for their genotyping by Polymerase Chain Reaction-Sequences Specific Olegionucleotide (PCR-SSO) technique. The present study revealed that diabetic patients were positively associated with HLA-DQB1*0201 (83% vs. 5.0%) which is the most prevalent in patients followed by DRB1*1137 (46.7% vs. 0.0%); DRB1*0401 (41.7% vs. 2.5%), and DRB1*1306(15% vs.0.0%) while HLA, A*0201;B*3559;Cw*0410 and DQB1*0501 is negatively associated in type 2DM in comparison to healthy control groups. This study has shown that there is no significant association between FBS, HbA1c, serum insulin, HOMA2 -function, HOMA2-IR, serum adiponectin, serum adenosine deaminase and HLA alleles