Immunochemistry of Solid-Phase Immunoassay de John E. Butler

Immunochemistry of Solid-Phase Immunoassay

John E. Butler

Immunochemistry of Solid-Phase Immunoassay fills a niché in the field of immunoassay and immunology. Although solid-phase immunoassay constitutes a major technology in biology and medicine, there is no comprehensive source devoted to the immunochemical principles involved. As a result, this book will benefit students, technicians, and researchers who use this technology, as well as immunodiagnostic and biotech companies who develop the technology. The book is not a methods manual; instead, it incorporates the concepts, data, and opinions of more than 25 investigators working in this field. Topics discussed include: the chemistry of solid-phases, the behavior or antibodies and antigens on solid phases, membrane solid-phases, reaction kinetics, antigen quantitation, enzyme systems, photophysics, immunochemical considerations in data analyses, multianalyte assays and occupancy concepts, antibody quantitation, streptavidin, a review of data analysis software, and solid-phase peptide immunoassay.

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Cuprins

General Principles: Perspectives, Configurations, and Principles (J.E. Butler). The Chemical Properties of Solid-Phases and Their Interaction With Proteins (P.J. Tarcha). Immunochemical Considerations in the Analysis of Data from Non-Competitive Solid-Phase Immunoassays (J.H. Peterman). Photophysics of Heterogeneous Immunoassays (O.S. Khahil). Enzyme Systems and Enzyme Conjugates for Solid-Phase ELISA (T.T. Ngo). Practical Applications: Competitive, Noncompetitive and Multianalyte Immunoassays (R.P. Ekins). Antigen Quantification: Measurement of Multivalent Antigens by Solid-Phase Immunoassay (R.G. Hamilton). Immunoblotting: Membrane Filters as the Solid Phase for Immunoassays (W.R. Brown, S.E> Dierks, J.E. Butler, and J.M. Gershoni). Quantitation of Specific Antibodies: methods of Expression, Standards, Solid-Phase Considerations, and Specific Applications (J.E> Butler and R.G. Hamilton). Critical Issues and Special Topics: The Specificity of Monoclonal Antibodies for Enzymes in Solution vs. Immobilized on Solid-Phases (L. Djavadi-Ohaniance and b. Friguet). Studies on the Covalent Attachment Antibodies to COntrolled Surfaces (P.D. Mize, A. Naqui, C.M. O'Connell, J.N. Waller, M. Fesler, R.G. Myatich, and W.E. Keating). The Application of Traditional Immunochemical Methods to Evaluate the Performance of Capture Antibodies Immobilized on Microtiter Wells (J.E. Butler, K.S. Joshi, and W.R. Brown). Considerations of the Interpretation of the Specificity of Monoclonal Antibodies Determined in Solid-Phase Immunoassays (F.J. Stevens). Monosized Particles as Solid-Phase for Immunoassays: Applications and Immunochemistry (K. Nustad, E. Paus, and O. Børmer). Enzyme Immunofiltration Assay for Human Creatine Kinase Isoenzyme MB (F. Poulsen and O.J. Bjerrum). The Use of Peptides as Solid-Phase Antigens (M. Lacroix, G. Dionne, M. Zrein, R.J. Dwyer, and R.J. Chalifour). Streptavidin: Purification, Utility, and Use in Antibody Immobilization (M. Suter). The Kinetics of Solid-Phase