In Situ Hybridization in Electron Microscopy
Autor Gerard Morel, Annie Cavalier, Lynda Williamsen Limba Engleză Hardback – 30 ian 2001
° hybridization on ultrathin sections of tissue embedded in hydrophilic resin (post-embedding method)
° hybridization prior to embedding (pre-embedding)
° hybridization on ultrathin sections of frozen tissue (frozen tissue method).
For each technique, the different stages are described in detail: the preparation of tissue, pretreatment, hybridization, and visualization of the hybridization products.
The book combines theory and practice, starting with the basic principles, then breaking down the experimental process into successive steps illustrated by numerous diagrams, detailed protocols, and tables. This is all done in a format that uses parallel columns to convey useful comments next to the theory and practical details alongside each stage of the protocol. Additionally, the summary tables provide the criteria for choosing the probe type and technique, and a detailed index aids in the search for information. in situ Hybridization In Electron Microscopy is an essential companion for applying these methods at the electron microscopic level.
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Specificații
ISBN-13: 9780849300448
ISBN-10: 0849300444
Pagini: 472
Ilustrații: 16 tables and 126 halftones
Dimensiuni: 178 x 254 x 28 mm
Greutate: 1.15 kg
Ediția:New.
Editura: CRC Press
Colecția CRC Press
ISBN-10: 0849300444
Pagini: 472
Ilustrații: 16 tables and 126 halftones
Dimensiuni: 178 x 254 x 28 mm
Greutate: 1.15 kg
Ediția:New.
Editura: CRC Press
Colecția CRC Press
Public țintă
Professional Practice & DevelopmentCuprins
General Introduction. Abbreviations. Probes. Principles of Methodology. Sample Preparation. Post-Embedding Technique. Pre-Embedding Technique. Frozen Tissue Technique. Semithin Sections. Controls and Problems. Examples of Results. Appendices. Glossary. Index.
Notă biografică
Morel, Gerard | Cavalier, Annie | Williams, Lynda
Descriere
In situ hybridization is a technique that allows for the visualization of specific DNA and RNA sequences in individual cells, and is an especially important method for studying nucleic acids in heterogeneous cell populations. in situ Hybridization in Electron Microscopy reviews the three main methods and describes the different stages in detail: th