Herbs have always been used as a common source of medicines, the Ficus benghalensis is an important herbal plant used in Ayurveda as a traditional medicinal system of India. In the present investigations, the preventive effect of Ficus benghalensis bark extract was evaluated against cyclophosphamide induced of chromosomal Abbreviation and micronucleus formation in the rat bone marrow cells. The single p.o administration of Ficus benghalensis bark ext at the dose of 250, 500 and 800 mg/kg body weight, 24 hours prior the administration of cyclophosphamide (at the dose of 50 mg/kg) have significantly prevented the micronucleus formations and Chromosomal Abbreviation in dose dependent manner in bone marrow cells of rat as compared to cyclophosphamide group. It is seems to have a preventive potential against CP-induced mutagenic effect in rat bone marrow cells.