Prodigiosin was isolated from fermented broth of isolated marine bacterial strain Vibrio sp. The compound was characterized, and evaluated for its antimicrobial properties. The isolated strain was molecularly identified based on 16S rRNA based phylogenetic analysis. This molecular analysis data suggested that this strain is closely related to Vibrio sp. The extract was purified and characterized by spectral data using NMR and MASS and confirmed as analogue 2,2'- 3-methoxy-1'amyl-5'-methyl-4-(1''-pyrryl)] Prodigiosin structure. Prodigiosin exhibited antioxidant and broad spectrum antimicrobial properties against gram negative and gram positive strains. Molecular docking study with different cancer targets revealed that this compound it may be a potential candidate at pharmaceutical sector.