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Introduction to Spatial Mapping of Biomolecules by Imaging Mass Spectrometry

Autor Bindesh Shrestha
en Limba Engleză Paperback – 28 apr 2021
Imaging mass spectrometry (MS) techniques are often utilized without an understanding of their underlying principles, making it difficult for scientists to determine when and how they can exploit MS to visualize their biomolecules of interest. Introduction to Spatial Mapping of Biomolecules by Imaging Mass Spectrometry is an essential reference to help scientists determine the status and strategies of biomolecule analysis, describing its many applications for diverse classes of biomolecules.
The book builds a foundation of imaging MS knowledge by introducing ionization sources, sample preparation, visualization guidelines, molecule identification, quantification, data analysis, etc. The second section contains chapters focused on case studies on analyzing a biomolecule class of molecules. Case studies include an introduction/background, and a summary of successful imaging MS studies with illustrative figures and future directions.


  • Provides the introductory foundations of imaging mass spectrometry for those new to the technique
  • Organized by topic to facilitate a quick deep dive, allowing researchers to immediately apply the imaging MS techniques to their work
  • Includes case studies summarizing the imaging MS techniques developed for the class of molecules
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Specificații

ISBN-13: 9780128189986
ISBN-10: 0128189983
Pagini: 270
Ilustrații: 125 illustrations (25 in full color)
Dimensiuni: 152 x 229 x 16 mm
Greutate: 0.37 kg
Editura: ELSEVIER SCIENCE

Public țintă

Scientists interested in imaging mass spectrometry, including bioanalytical chemists in academia and pharma, with or without experience with mass spectrometry (either as mass spec lab scientists/PIs or customers of such mass spec services). Biochemists, biologists, biomedical scientists or other scientists who want to learn more about visualizing specific biomolecules on the tissue.

Cuprins

1. Fundamentals of Imaging Mass Spectrometry2. Ionization Source for Imaging Mass Spectrometry3. Sample Preparation and MALDI Matrix Applications4. Tissue Sectioning for Imaging Mass Spectrometry5. Matrix for Matrix-assisted Laser Desorption/Ionization (MALDI)6. Molecule Identification Approaches in Imaging Mass Spectrometry7. Quantitation Workflow in Imaging Mass Spectrometry8. Spatial Resolution of Imaging Mass Spectrometry9. Visualization of Imaging Mass Spectrometry10. Analysis of imaging mass spectrometry data11. Multimodal imaging mass spectrometry12. Validation and Standardization of Imaging Mass Spectrometry13. Towards Clinical Imaging Mass Spectrometry14. Case study: Imaging N-Glycan on tissue15. Case study: Reactive matrix and neurotransmitters and brain metabolites16. Case study: Endogenous metabolites in mammalian tissues17. Case study: Imaging of small drug molecules18. Case study: Imaging Gangliosides