Introductory Experiments on Biomolecules and their Interactions
Autor Robert K. Delong, Qiongqiong Zhouen Limba Engleză Paperback – 21 mai 2015
The text parallels lectures using a standard biochemistry undergraduate text. Unlike most current lab manuals available in the market which simply emphasize an introduction of techniques, this lab manual provides students with opportunities to demonstrate and prove the knowledge and theories they learn from class.
- Features quantitative analysis of RNA degradation by RNase
- Contains problem sets, calculations, and references for each lab fully immersing students in the learning process
- Includes instruction on how to maintain a lab notebook and write a formal lab report
- Provides hands-on engagement with the four major types of biomolecules and “real-life and better applied examples of molecular interactions
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Specificații
ISBN-13: 9780128009697
ISBN-10: 0128009691
Pagini: 126
Ilustrații: 20 illustrations
Dimensiuni: 152 x 229 x 8 mm
Greutate: 0.23 kg
Editura: ELSEVIER SCIENCE
ISBN-10: 0128009691
Pagini: 126
Ilustrații: 20 illustrations
Dimensiuni: 152 x 229 x 8 mm
Greutate: 0.23 kg
Editura: ELSEVIER SCIENCE
Cuprins
Foreword and IntroductionLaboratory safety policies, notebook, lab report formatting & other expectationsExperiment 1: An introduction to basic math and operations in the biomolecular laboratory Experiment 2: Buffer preparation and pH Experiment 3: Amino acid properties and an introduction to chromatography Experiment 4: Rapid purification, gel electrophoresis and enzyme activity assay of the firefly protein enzyme luciferase Experiment 5: Hexokinase and G6DH catalyzed reactions of glucose measurementExperiment 6: Polymerase chain reaction and DNA sequence analysisExperiment 7: Investigating protein:nucleic acid interactions by electrophoretic mobility shift assay (emsa)Experiment 8: RNA quantification and degradation kineticsExperiment 9: Microscale preparation of liposome under mock biotechnology conditions: testing its protein interaction Experiment 10: Preparation and characterization of a fluorescently tagged liposome-protein-DNA particle as a simple model “artificial cell