Deafness is a hearing impairment and can be classified as genetic or acquired, pre-lingual or post-lingual and syndromic or non-syndromic. This book contains details of materials, methods, literature,results and other information which will enable to study the linkage of genetic deafness. Consanguineous families of different ethnic groups having deaf individuals were studied. Pedigrees were drawn of all families having three or more affected members and showed recessive mode of inheritance. DNA was extracted from blood samples. Short Tandem Repeat markers for DFNB3 locus were amplified using PCR and genotyped for identification of hereditary hearing loss due to DFNB3 in Pakistani families.