Manual of Practical Medical Biochemistry

1. General
General Instructions
Laboratory Equipments and Procedures
Buffers and pH
Quality Control
2. Analysis of Urine and Cerebrospinal Fluid
Normal Urine: Characteristics and Analysis
Abnormal Constituents of Urine
Screening of Urine for Inborn Errors of Metabolism and the Use of Paper Chromatography
Composition of Cerebrospinal Fluid
3. Quantitative Experiments
Principles of Colorimetry
Principles of Spectrophotometer
Estimation of Glucose
Glucose Tolerance Test
Estimation of Serum and Urine Creatinine: Creatinine Clearance
Estimation of Urea
Serum Proteins—Albumin: Globulin Ratio
Serum Total Cholesterol
Lipid Profile
Serum Calcium and Phosphorus
Serum Bilirubin
Serum Transaminases
Serum Alkaline Phosphatase
Serum Uric Acid
4. Equipments And Procedures
Point of Care Testing
pH Meter
Paper Chromatography and Thin Layer Chromatography
Protein Electrophoresis
Polyacrylamide Gel Electrophoresis
Electrolyte Analysis
Arterial Blood Gas Analyzer
Enzyme-Linked Immunosorbent Assay
Immunodiffusion
Autoanalyzer
DNA Isolation from Blood/Tissues
5. Function Tests
Gastric Function Tests and Analysis of Gastric Juice
Pancreatic Function Tests
Liver Function Tests
Analysis of Bile
Renal Function Tests
Thyroid Function Tests
Adrenal Function Tests
6. Food and Energy
Energy Content of Food and Glycemic Index
Fats in Food
7. Clinical Case Studies
Basis and Rationale of Biochemical Tests Done in Certain Clinical Conditions
8. Objective Structured Practical Examination
Objective Structured Practical Examination
9. Reagents
Reagent Preparation
10. Normal Values
Normal Values
Worksheet

Evangeline Jones MD
Formerly Professor and Head, Department of Biochemistry, Vinayaka Missions Kirupanandha, Variyar Medical College and Government Mohan Kumaramangalam Medical College Salem, Tamil Nadu, India