Glacier Bay National Park and Preserve (GBNPP), Alaska, like many pristine high latitude areas, is exposed to atmospherically deposited contaminants such as mercury (Hg). Although the harmful effects of Hg are well established, information on this contaminant in southeast Alaska is scarce. Here, we assess the level of this contaminant in several aquatic components (water, sediments, and biological tissue) in three adjacent, small streams in GBNPP that drain contrasting landscapes but receive similar atmospheric inputs: Rink Creek, Salmon River, and Good River.