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Methods in Cilia and Flagella: Methods in Cell Biology, cartea 127

Renata Basto, Wallace F. Marshall
en Limba Engleză Hardback – 25 mar 2015
The goal of this book is to collect methods and protocols for studying cilia in a wide range of different cell types, so that researchers from many fields of biology can start exploring the role of cilia in their own system.


  • Chapters are written by experts in the field
  • Cutting-edge material
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Specificații

ISBN-13: 9780128024515
ISBN-10: 0128024518
Pagini: 614
Dimensiuni: 191 x 235 x 33 mm
Greutate: 1.47 kg
Editura: ELSEVIER SCIENCE
Seria Methods in Cell Biology


Cuprins

1. Imaging centrosomes and cilia in the mouse kidney
Masato Hoshi, Jinzhi Wang, Sanjay Jain and Moe R. Mahjoub
2. Ependymal cell differentiation, from monociliated to multiciliated cells
Nathalie Delgehyr, Alice Meunier, Marion Faucourt, Montserrat Bosch Grau, Laetitia Strehl, Carsten Janke, Nathalie Spassky
3. Observing planar cell polarity in multiciliated mouse airway epithelial cells
Eszter K. Vladar, Yin Loon Lee, Tim Stearns and Jeffrey D. Axelrod
4. Imaging and analysing primary cilia in cardiac cells
Nicolas Diguet, Jean-François Le Garrec, Tommaso Lucchesi and Sigolène M. Meilhac
5. Cilia in photoreceptors
Linjing Li, Manisha Anand, Kollu Nageswara Rao and Hemant Khanna
6. Analysis of primary cilia in the developing mouse brain
Judith TML Paridaen, Wieland B Huttner and Michaela Wilsch-Bräuninger
7. In vivo investigation of cilia structure and function using Xenopus
Eric R. Brooks and John B. Wallingford
8. A quantitative approach to study endothelial cilia bending stiffness during blood flow mechanodetection in-vivo
Francesco Boselli, Jacky G. Goetz, Gilles Charvin, Julien Vermot
9. Quantitative description of fluid flows produced by left-right cilia in zebrafish
Craig Fox, M. Lisa Manning and Jeffrey D. Amack
10. Efficient live fluorescence imaging of intraflagellar transport in mammalian primary cilia
Hiroaki Ishikawa and Wallace F. Marshall
11. Analysis of soluble protein entry into primary cilia using semi-permeabilized cells
David K. Breslow and Maxence V. Nachury
12. Methods for imaging individual cilia in living echinoid embryos
Robert L. Morris, Hans W. Pope, Adam N. Sholi, Leah M. Williams, Chelsea R. Ettinger, Gwendolyn M. Beacham, Tatsushi Shintaku, Zachary D. Abbott, Elyse M. Doherty
13. The planarian Schmidtea mediterranea as a model for studying motile cilia and multiciliated cells
Cyril Basquin, Anne-Marie Orfila and Juliette Azimzadeh
14. Quantitative analysis of flagellar proteins in Drosophila sperm tails
Teresa Mendes Maia, Perrine Paul-Gilloteaux, Renata Basto
15. Imaging cilia in Drosophila melanogaster
Jennifer Vieillard, Jean-Luc Duteyrat, Elisabeth Cortier, Bénédicte Durand
16. Studying G-protein-coupled receptors: Immunoblotting, immunoprecipitation, phosphorylation, surface labeling, and cross-linking protocols
Kasturi Pal, Hemant Badgandi, and Saikat Mukhopadhyay
17. Image analysis of Caenorhabditis elegans ciliary transition zone structure, ultrastructure, molecular composition and function
Anna A. W. M. Sanders, Julie Kennedy and Oliver E. Blacque
18. Genetic and genomic approaches to identify genes involved in flagellar assembly in Chlamydomonas reinhardtii
Huawen Lin and Susan K. Dutcher
19. Axonemal motility in Chlamydomonas
Ken-ichi Wakabayashi and Ritsu Kamiya