The study of in-vitro micropropagation has assumed enormous importance with the tremendous pace of progress in different disciplines of biological sciences. The tissue culture will play an important role in solving the problems of conventional methods for propagation, hybridization, embryo rescue, production of secondary metabolites, production of virus free plants and paternity disputes. This book help the orchid grower to keep themselves abreast of the latest developments along with methods of mass propagation through various explants as well as conservation of endangered and rare orchids. This book also provide the technique for horticulturist those carries commercial purpose. This book will cater to the immediate needs of students, researcher, faculty members and horticultural industries.