Molecular Biology LabFax: Gene Analysis: LabFax, cartea 2
Autor T. A. Brownen Limba Engleză Hardback – 5 iul 1998
- Provides most up-to-date information on techniques, sources, and equipment for anyone using molecular biological techniques
- Clearly presented information
- Divided into two parts which together comprise a complete collection of molecular biological methods
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Specificații
ISBN-13: 9780121361105
ISBN-10: 0121361101
Pagini: 255
Dimensiuni: 171 x 248 x 20 mm
Greutate: 0.68 kg
Ediția:Revised.
Editura: ELSEVIER SCIENCE
Seria LabFax
ISBN-10: 0121361101
Pagini: 255
Dimensiuni: 171 x 248 x 20 mm
Greutate: 0.68 kg
Ediția:Revised.
Editura: ELSEVIER SCIENCE
Seria LabFax
Public țintă
AUDIENCE: Research scientists at the postgraduate, postdoctoral level and above working in molecular biology, recombinant DNA, gene cloning, as well as other areas within the life sciences.Cuprins
Abbreviations.
Nucleic Acid Blotting and Hybridization:
Membranes for Nucleic Acid Blotting.
Factors Influencing Hybridization.
Synthetic Oligonucleotide Probes.
Reagents and Conditions for Nucleic Acid Hybridization.
DNA Sequencing:
Chain Termination Sequencing.
Chemical Degradation Sequencing.
Denaturing Polyacrylamide Gel Electrophoresis.
Troubleshooting.
DNA Databases and Software for Sequence Analysis.
Polymerase Chain Reaction:
Amplification Methodology.
Cloning PCR Products.
Sequencing PCR Products.
Troubleshooting.
Labeling Nucleic Acids:
Radiochemistry.
Labeling Procedures.
Detection of Radioactivity.
Protection from Radiochemical Hazards.
Non-Radioactive Labeling.
Agarose Gel Electrophoresis:
Agarose Gels.
Electrophoresis Systems.
DNA and RNA Markers for Agarose Gels.
Centrifugation:
Calculation of the Relative Centrifugal Force.
Density Gradient Centrifugation.
Care of Rotors.
Chemicals and Reagents:
Details of Individual Compounds.
Safety:
Protection from Chemical Hazards.
Protection from Microbiological Hazards.
Nucleic Acid Blotting and Hybridization:
Membranes for Nucleic Acid Blotting.
Factors Influencing Hybridization.
Synthetic Oligonucleotide Probes.
Reagents and Conditions for Nucleic Acid Hybridization.
DNA Sequencing:
Chain Termination Sequencing.
Chemical Degradation Sequencing.
Denaturing Polyacrylamide Gel Electrophoresis.
Troubleshooting.
DNA Databases and Software for Sequence Analysis.
Polymerase Chain Reaction:
Amplification Methodology.
Cloning PCR Products.
Sequencing PCR Products.
Troubleshooting.
Labeling Nucleic Acids:
Radiochemistry.
Labeling Procedures.
Detection of Radioactivity.
Protection from Radiochemical Hazards.
Non-Radioactive Labeling.
Agarose Gel Electrophoresis:
Agarose Gels.
Electrophoresis Systems.
DNA and RNA Markers for Agarose Gels.
Centrifugation:
Calculation of the Relative Centrifugal Force.
Density Gradient Centrifugation.
Care of Rotors.
Chemicals and Reagents:
Details of Individual Compounds.
Safety:
Protection from Chemical Hazards.
Protection from Microbiological Hazards.
Recenzii
"The book is compiled with care and contains text that explains technical terms that then can be used with understanding...especially useful for young investigators who are new to the field...this book qualifies to be the present-day standard reference..."—Amit Gupta, University of Illinois, in ASM NEWS (1999)