Genetic analysis of isolated DNA from hydatid cysts collected from human and animals was done by Polymerase Chain Reaction (PCR) to determine genetic variation depending on Random Amplified Polymorphic DNA . In the present study 10 primers have been used, during which the genetic variations were revealed among isolated (extracted DNA) of hydatid cysts which was collected from human and other intermediate hosts . The current results of this study have shown the following : 1 - It was found one primer (OPA ¿ 01) was able to diagnose sample numbered 1 which represent the isolated DNA of liver hydatid cyst which was obtained from human at age group 10 ¿ 20 years old . 2 - The ability of primer OPC ¿ 10 to determine fingerprinting of DNA sample of Sheep liver hydatid cyst . 3 - The ability of primer OPC ¿ 05 to determine fingerprinting of DNA sample of human spleen hydatid cyst which was obtained from human at age group 30 ¿ 40 years old . 4 - The ability of primer OPE ¿ 07 to determine fingerprinting of DNA sample of Goat liver hydatid cyst .