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Molecular Diagnostics in Cytopathology: A Practical Handbook for the Practicing Pathologist

Editat de Sinchita Roy-Chowdhuri, Paul A. VanderLaan, John M. Stewart, Gilda da Cunha Santos
en Limba Engleză Paperback – 17 dec 2018
Molecular diagnostics are increasingly used to help guide targeted therapy in solid organ tumors and hematologic malignancies. A large proportion of molecular testing is performed on limited-volume samples obtained via minimally invasive techniques, such as fine needle aspiration. Increasingly, cytopathologists play an essential role in this process, both in the triage of specimens during rapid on-site evaluation and in the evaluation of archival samples to determine suitability for ancillary testing. Therefore, it is imperative that practicing cytopathologists stay abreast of up-to-date diagnostic, prognostic, and predictive ancillary tests that can be used on limited cytologic material. This is a challenge since the landscape of known genomic alterations is constantly evolving and the subsequent set of testing options is ever expanding.

The proposed text will provide a user-friendly quick-reference handbook to serve as a useful resource for practicing pathologistsand laboratory personnel dealing with, and interested in, this evolving field of molecular cytopathology. Essential components to be presented include: 1) pre-analytic factors that affect sample selection and evaluation; 2) specimen preparation to maximize confidence in results; 3) interpretation of results; 4) potential limitations; and 5) workflow algorithms.  In addition, specific disease specific molecular testing details will be outlined to provide the reader with resources for quick reference. All chapters will be written by experts in their fields and will include the most up-to-date scientific and clinical information.

Molecular Diagnostics in Cytopathology will be of value to Cytopathologists, Cytotechnologists, Cytotechnology students, Cytopathology fellows, Surgical pathologists, Pathology residents and fellows, Molecular Pathologists, Molecular pathology fellows, Molecular technologists, as well as  Translational researchers with an interest in molecular cytopathology.
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Specificații

ISBN-13: 9783319973968
ISBN-10: 3319973967
Pagini: 697
Ilustrații: XV, 534 p. 93 illus., 91 illus. in color.
Dimensiuni: 127 x 203 x 24 mm
Greutate: 0.95 kg
Ediția:1st ed. 2019
Editura: Springer International Publishing
Colecția Springer
Locul publicării:Cham, Switzerland

Cuprins

Introduction:  Overview of Current Molecular Diagnostic Testing on Cytology Samples.- The Cytology Specimen and Preparations: Advantages and Limitations.- Sample Acquisition and Test Requisition.- Pre-Analytic Workflow and Specimen Evaluation.- DNA-Based Sequencing Assays.- RNA-Based Assays.- FISH Testing of Cytology Specimens: Pre-Analytic, Analytic and Post-Analytic Considerations.- Molecular Cytopathology Correlations: Interpretation of Molecular Diagnostic Results.- Human Papillomavirus (HPV) Testing of Head and Neck Cancers.- Human Papillomavirus (HPV) Testing on Cervical Cytology Specimens.- Molecular Diagnostics in Lung Cytology.- Molecular Diagnostics in Thyroid Cytology.- Molecular Diagnostics in Breast Cytology.- Molecular Diagnostics in Salivary Gland Cytology.- Molecular Diagnostics in Pancreatic and Biliary Cytology.- Fluorescence In Situ Hybridization (FISH) Testing in Urinary Tract Cytology.- Molecular Diagnostics in Hematological Malignancies.- Molecular Diagnostics in Bone and Soft Tissue Tumors.- Molecular Diagnostics in Pediatric Cytopathology.- Molecular Cytopathology: Final Thoughts and Future Directions.

Notă biografică

Sinchita Roy-Chowdhuri
The University of Texas MD Anderson Cancer Center
Houston, TX
USA

Paul A. VanderLaan 
Beth Israel Deaconess Medical Center
Harvard Medical School
Boston, MA
USA

John M. Stewart
The University of Texas MD Anderson Cancer Center
Houston, TX
USA

Gilda da Cunha Santos
Sunnybrook Health Science Center
Toronto, Ontario
Canada

Textul de pe ultima copertă

The main focus of this book is to provide an overview of the principles of molecular diagnostics in context of cytopathology specimens together with a basic understanding and working knowledge of the available technology, platforms, and clinical applications. The initial sections of the book summarize the pre-analytic aspects of molecular testing, including cytology specimen preparation and handling, specimen selection and evaluation, and workflow algorithms as well as the analytic considerations for a variety of nucleic acid and protein-based testing. The remaining section focuses on disease specific molecular testing for various organ-based applications. 

Molecular Diagnostics in Cytopathology: A Practical Handbook for the Practicing Pathologist serves as a useful resource for cytopathologists, surgical pathologists, molecular technologists, and all others dealing with the evolving field of molecular cytopathology. 


Caracteristici

Provide a user-friendly quick-reference handbook Written by experts in their fields Richly illustrated with color micrographs, illustrations, and tables