Molecular Methods for Microbial Identification and Typing de K.J. Towner

Molecular Methods for Microbial Identification and Typing

K.J. Towner

A. Cockayne

en Limba Engleză Paperback – 30 sep 1993

The accurate identification and typing of microbes is essential for workers active in all fields of microbiology. Many examples of modern molecular methods have been concealed in scientific and medical literature but this introductory text considers the possible applications of such methods and compares their advantages and disadvantages.

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Cuprins

1 An introduction to microbial identification and typing methods.- 1.1 A brief overview of microbial identification.- 1.2 Conventional biological methods for microbial typing.- 1.3 Molecular approaches to microbial identification and typing.- References.- 2 Analysis of nucleic acid profiles.- 2.1 Introduction.- 2.2 Plasmid analysis.- 2.3 Analysis of chromosomal DNA.- 2.4 Summary.- References.- 3 Identification and typing by nucleic acid hybridization techniques.- 3.1 Introduction.- 3.2 Basic principles of nucleic acid hybridization.- 3.3 Analysis of RFLPs by hybridization techniques.- 3.4 Summary.- References.- 4 Nucleic acid amplification and sequencing techniques.- 4.1 Introduction.- 4.2 The polymerase chain reaction (PCR).- 4.3 Other amplification methods.- 4.4 Nucleic acid sequencing techniques.- 4.5 Summary.- References.- 5 Analysis of protein and lipopolysaccharide profiles.- 5.1 Introduction to protein fingerprinting.- 5.2 Protein analysis.- 5.3 Lipopolysaccharide typing schemes.- 5.4 Summary.- References.- 6 Typing and identification of microorganisms with antibodies.- 6.1 Introduction.- 6.2 Production of antibodies for serotyping and identification.- 6.3 Techniques used in serotyping.- 6.4 Summary.- References.- 7 Final summary and future prospects.

Recenzii

The value of this book is seen above all in a critical reviewing of the advantages and disadvantages of the techniques considered. The text is supplemented by numerous references to literature and illustrations. This book provides the reader with a detailed survey of the subject and a wealth of suggestions. - Zentralblatt fur Bakteriologie