Molecular Microbial Ecology: Advanced Methods
Editat de Mark Osborn, Cindy Smithen Limba Engleză Paperback – 11 aug 2005
Molecular Microbial Ecology gives a comprehensive guide to the recent revolution in the study of microorganisms in the environment. Details are given on molecular methods for isolating some of the previously uncultured and numerically dominant microbial groups. PCR-based approaches to studying prokaryotic systematics are described, including ribosomal RNA analysis and stable isotope probing. Later chapters cover DNA hybridisation techniques (including fluorescent in situ hybridisation), as well as genomic and metagenomic approaches to microbial ecology. Gathering together some of the world’s leading experts, this book provides an invaluable introduction to the modern theory and molecular methods used in studying microbial ecology.
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Specificații
ISBN-13: 9781859962831
ISBN-10: 1859962831
Pagini: 400
Dimensiuni: 178 x 254 x 23 mm
Greutate: 0.7 kg
Ediția:1
Editura: CRC Press
Colecția Taylor & Francis
Seria Advanced Methods
ISBN-10: 1859962831
Pagini: 400
Dimensiuni: 178 x 254 x 23 mm
Greutate: 0.7 kg
Ediția:1
Editura: CRC Press
Colecția Taylor & Francis
Seria Advanced Methods
Public țintă
Postgraduate and UndergraduateCuprins
1. Nucleic Acid Extraction From Environmental Samples. 2. Prokaryotic Systematics: PCR And Sequence Analysis Of Amplified 16S rRNA Genes. 3. DNA Fingerprinting Of Microbial Communities. 4. Molecular Typing Of Environmental Isolates. 5. RT-PCR And mRNA Expression Analysis Of Functional Genes. 6.Quantitative Real-Time PCR. 7. Stable Isotope Probing. 8. Applications Of Nucleic Acid Hybridization In Microbial Ecology. 9. Fluorescence in situ Hybridisation For The Detection Of Prokaryotes. 10. Lessons From The Genomes: Microbial Ecology And Genomics. 11. Metagenomic Libraries From Uncultured Microorganisms. 12. A Molecular Toolbox For Bacterial Ecologists: PCR Primers For Functional Gene Analysis. 13. Molecular Detection Of Fungal Communities In Soil. 14. Environmental Assessment: Bioreporter Systems. 15. Bioinformatics And Web Resources For The Microbial Ecologist.
Notă biografică
Dr A. Mark Osborn-Department of Animal & Plant Sciences, University of Sheffield
Descriere
Molecular Microbial Ecology provides both a theoretical and a methodological introduction to various key molecular techniques used in microbial ecology, focusing particularly on nucleic acid-based methodologies.